Bioresource Technology

Volume 284, July 2019, Pages 240-247
Bioresource Technology

Fermentation quality and microbial community of alfalfa and stylo silage mixed with Moringa oleifera leaves

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High moisture alfalfa and stylo ensiled with M. oleifera leaves (MOL).

MOL improved fermentation quality of alfalfa and stylo silage.

MOL decreased the relative abundance of Clostridium from 23% to 0.2%.

MOL increased the relative abundance of Lactobacillus from 42% to 86%.

MOL decreased proteolysis in alfalfa and stylo silage.


The silage quality of alfalfa and stylo without or with 25%, 50% Moringa oleifera leaves (MOL) was investigated, and microbial community after ensiling was analysed. Results showed that the silage samples with MOL have lower butyric acid (0.50 vs 1.20, 0.60 vs 14.5 g/kg dry matter (DM) in alfalfa and stylo silage, respectively), ammonia-N (152 vs 262, 109 vs 180 g/kg total N) content and DM loss (7.71% vs 14.6%, 6.49% vs 18.9%). The addition of MOL also influenced the bacterial community distribution. The relative abundance of Enterobacter decreased from 58.6% to 30.5%, 17.4% to 9.1% in alfalfa and stylo silage when 50% MOL was added. Clostridium decreased from 23.5% to 0.2% in stylo silage, whereas Lactobacillus abundance increased from 30.4% to 49.9%, 41.8% to 86.0% in alfalfa and stylo silage, respectively. In conclusion, mixing with MOL could be a feasible way to improve the quality of alfalfa and stylo silage.


Fermentation quality
Microbial community
Mixed ensiling
Moringa oleifera

C.W. and L.H. contributed equally to this article.

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